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Temperature micro-Raman
study of lysozyme crystals
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Vasylkiv Yu., Polomska M., Nastishin Yu. and
Vlokh R.
Keywords: Raman spectra, biocrystals, lysozyme
crystals, phase transitions
PACS: 87.64.Je, 78.30.Jw
Ukr. J. Phys. Opt. 8 158-165
doi: 10.3116/16091833/8/3/158/2007
Received: 13.06.2007
We have performed for the first time micro-Raman
studies of lysozyme crystals for the temperatures lower than the room one
(in the range 282-300K). Anomalous temperature dependence of the
positions of Raman scattering bands has been detected and discussed, which
corresponds to the crystalline lysozyme structure in the vicinity of
T=285K.
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REFERENCES
1. Jolles P and Berthou J, 1972. High temperature crystallization of
lysozyme: an example of phase transition. FEBS Lett. 23: 22-23.
doi:10.1016/0014-5793(72)80273-4
http://dx.doi.org/10.1016/0014-5793(72)80273-4
2. Berthou J and Jolles P, 1974. A phase transition in protein crystal:
the example of hen lysozyme. Biochim. et Biophys. Acta. 336: 222-227.
3. Kobayashi J, Asahi T, Sakurai M, Kagomiya I, Asai H and Asami H,
1998. The optical activity of lysozyme crystals. Acta Cryst. A54, 581-590.
doi:10.1107/S0108767398001986
http://dx.doi.org/10.1107/S0108767398001986
4. Svanidze AV, Lushnikov SG and Seiji Kojima, 2006. Anomalous temperature
behavior of hypersonic acoustic phonons in a lysozyme crystal. JETP Lett.
84: 551-555.
doi:10.1134/S002136400622005X
http://dx.doi.org/10.1134/S002136400622005X
5. Vasylkiv Yu, Nastishin Yu and Vlokh R, 2007. On the problem of phase
transitions in lysozyme crystals. Ukr. J. Phys. Opt. 8: 83-87.
doi:10.3116/16091833/8/2/83/2007
http://dx.doi.org/10.3116/16091833/8/2/83/2007
6. Teslyuk I, Vasylkiv Yu, Nastishin Yu and Vlokh R, 2007. Structural
phase transition in lysozyme crystals. Ferroelectrics. 346: 49-55.
doi:10.1080/00150190601180216
http://dx.doi.org/10.1080/00150190601180216
7. Teslyuk I, Nastishin Yu and Vlokh R, 2004. Structural phase transition
in lysozyme single crystals. Ukr. J. Phys. Opt. 5: 118-122.
doi:10.3116/16091833/5/4/118/2004
http://dx.doi.org/10.3116/16091833/5/4/118/2004
8. Blanch EW, Hecht L and Barron LD. 2003. Vibrational Raman optical
activity of proteins, nucleic acids, and viruses. Methods 29: 196–209.
doi:10.1016/S1046-2023(02)00310-9
http://dx.doi.org/10.1016/S1046-2023(02)00310-9
9. Hisako Urabe, Yoko Sugawara, Mitsuo Ataka and Allan Rupprecht. 1998.
Low-Frequency Raman Spectra of Lysozyme Crystals and Oriented DNA Films:
Dynamics of Crystal Water. Biophys. J. 74: 1533–1540
10. Hédoux A, Ionov R, Willart J-F, Lerbret A, Affouard F, Guinet
Y, Descamps M, Prévost D, Paccou L and Danéde F, 2006. Evidence
of a two-stage thermal denaturation process in lysozyme: A Raman scattering
and differential scanning calorimetry investigation. J. Chem. Phys. 124:
014703-1-7.
11. Ionov R, He’doux A, Guinet Y, Bordat P, Lerbret A, Affouard F,
Prevost D and Descamps M, 2006. Sugar bioprotective effects on thermal
denaturation of lysozyme: Insights from Raman scattering experiments and
molecular dynamics simulation J. Non-Cryst. Sol. 352: 4430 –4436
12. Razumas V, Talaikyte Z, Barauskas J, Larsson K, Miezis Y and Nylander
T, 1996. Effects of distearoylphosphatidylglycerol and lysozyme on the
structure of the monoolein-water cubic phase: X ray diffraction and Raman
scattering studies. Chem. and Phys. of Lipids. 84: 123-138.
doi:10.1016/S0009-3084(96)02629-1
http://dx.doi.org/10.1016/S0009-3084(96)02629-1
13. Lu J, Cow P-S and Carpenter K, 2003. Phase transitions in lysozyme
solutions characterized by differential scanning calorimetry. Prog. Cryst.
Growth Charact. Mat. 46: 105-129.
doi:10.1016/S0960-8974(03)90004-0
http://dx.doi.org/10.1016/S0960-8974(03)90004-0
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