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Spectral Characteristics of Hemoglobin Taken from the
Blood of Rats Subjected to Durable Ethanol Intoxication
1,2Dudok K.P., 1Vlokh R., 1,3Vlokh
I., 1Dudok T., 2Fedorovich A.M., 1,4Moroz
O.M., 1,4Grinchishin N.M.
1Institute of Physical Optics, 23 Dragomanov
St., 79005 Lviv, Ukraine
2Biochemical Department, Lviv National
University, 4 Hrushevsky St., 79005 Lviv, Ukraine
3Department of Psychiatry, Psychology
and Sexology, State Medical University, 69 Kulparkivska St., 79010 Lviv,
Ukraine
4State Medical University, Scientific
Research Laboratory, 69 Pekarska St., 79010 Lviv, Ukraine
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We have studied the conformation state of the hemoglobin taken from
the blood of laboratory rats of four generations that suffered durable
ethanol intoxication. The rats have been intoxicated with adding 15% aqueous
ethanol solution to their ration, instead of water. The colouring agents,
Coomassi G-250 and Cibacron Blue F3G-A, have been used as probes. The obtained
results show definite differences in the hemoglobin absorption spectral
maxima for the control and intoxicated groups of animals. It has been found
that the absorption band centred at the wavelength 566,8nm is available
for the complexes Coomassi G-250?CNMetHb of the control group of rats.
In case of the same complexes for the intoxicated rats, the maximum corresponds
to the wavelength in range of 571.4?580.7nm. The Cibacron-MetHb complex
is characterized with an intensive band at 624-628nm. It has been shown
that intensification of hypo-chromic effect depends on the distance of
ethanol-intoxicated (EI) posterity from their parents. The mechanisms for
the appearance of new hemoglobin fraction in the EI rats’ generation have
been discussed.
Key words: optical spectroscopy, alcohol intoxication, hemoglobin,
methemoglobin, cyan-methemoglobin
PACS: 42.66.-p, 33.55.-b
doi 10.3116/16091833/5/3/104/2004 |
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