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Comparison of the Blood Component Optical Transmission
Spectra of White Rats with and without Ethanol Intoxication
1Dudok T., 1Korobova O., 1Korobov
V., 2Moroz O., 3Vlokh I., 1Vlokh R.
1Institute of Physical Optics, 23 Dragomanov
Str., 79005 Lviv, Ukraine
2Scientific Research Laboratory, National
Medical University, 69 Pekarska Str., 79010 Lviv, Ukraine
3Department of Psychiatry, Psychology and
Sexology, National Medical University, 96 Kulparkivska Str., 79010 Lviv,
Ukraine
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In this report we present spectroscopic transmission studies of hemoglobin
extracted from the vein blood of laboratory rats free of and exposed to
ethanol intoxication, which are performed in order to analyze the relevant
spectral differences. The animals were divided into the intoxicated group
(IG) and the control group (CG). The results show a noticeable difference
of the absorption/transmission spectra of hemoglobin extracted from the
blood of these two groups. The difference in the absorption spectra is
probably associated with the fact that the vein blood of ethanol-intoxicated
animals includes more RHb than that of the CG. The deoxygenation process
rate for the hemoglobin of intoxicated animals reaches a higher value and
is quicker than that for the CG. It means that the affinity of the IG hemoglobin
to oxygen is smaller than that for the CG. The minimum in the transmission
spectra of hemoglobin with the dissolved cibacron blue colouring agent
is shifted to a longer wavelength range (620 nm). The transmittance value
for the IG is smaller than that for the CG, implying probably that the
immunoglobulin G (IgG) in the IG possesses a more essential decomposition
of its spatial structure, when compare with the CG. The changes in the
transmission spectra of hemoglobin are detected after adding H2O2 and bromthymol
blue, indicating the protein conformational changes for the IG animals.
Key words: transmission spectra, hemoglobin, ethanol intoxication,
white rats.
PACS: 42.66.-p, 33.55.-b
doi 10.3116/16091833/4/3/119/2003 |
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